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-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer
ARP-161 is a human T-cell line, derived from the HUT 78 cell line, infected with non-cloned, non-plaque-purified Simian immunodeficiency virus (SIV). The virus was isolated from a Macaca fascicularis found to be infected at the New England Regional Primate Center.
Note: The virus grows best in CEM×174 cells.
Growth Characteristics: Cells grow as a suspension. Maintain cultures at 0.5 × 106 to 1 × 106 per mL and split twice per week at a ratio of 1:3 to 1:4, and monitor for virus production. Virus production is dramatic when first reseeded, then decreases. Aliquots of early passage supernatant should be saved and stored in liquid nitrogen. When virus production decreases, mix uninfected CEM×174 cells with infected cells at a ratio of 3:1 or 4:1. Virus production will increase in 7 to 10 days. Alternately, a thawed 1 mL aliquot of early passage virus can be used to infect 6 mL of 0.5 × 106 to 1 × 106 CEMx174 cells per mL.
Growth Medium: RPMI 1640 supplemented with 10% fetal bovine serum, 100 U per mL penicillin and 100 µg per mL streptomycin ARP-161 is negative for mycoplasma, bacteria and fungi. Each vial of ARP-161 contains approximately 9 x 106 cells in RPMI 1640 supplemented 10% fetal bovine serum and 10% dimethyl sulfoxide (DMSO). Please refer to the appropriate data sheet for lot-specific information.
Daniel, M. D., et al. “Isolation of T-Cell Tropic HTLV-III-Like Retrovirus from Macaques.” Science 228 (1985): 1201-1204. PubMed: 3159089.
Kestler, H. W. 3rd, et al. “Comparison of Simian Immunodeficiency Virus Isolates.”Nature 331 (1988): 619-622. PubMed: 2893293.
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